高原牧区藏绵羊与兰州大尾羊颌下腺组织学特征比较

doi:10.11843/j.issn.0366-6964.2019.10.019

摘要/Abstract

摘要： 通过比较高原牧区低氧环境下藏绵羊与大尾羊颌下腺组织学结构的异同及EGF、EGFR、eNOS的表达特点，为分析反刍动物颌下腺生理机能提供形态学参考。应用特殊染色方法、免疫组织化学SP法及免疫荧光法观察藏绵羊和大尾羊颌下腺组织结构特点及EGF、EGFR、eNOS的定位与分布特征。结果显示：藏绵羊颌下腺浆液性腺泡比例大于兰州大尾羊。AB、PAS、AB-PAS在黏液性腺泡及混合性腺泡阳性表达，浆液性腺泡无表达。免疫组织化学显示，EGF在藏绵羊颌下腺纹状管、闰管上皮细胞及浆液性腺泡表达量显著低于大尾羊，EGFR在藏绵羊颌下腺浆液性腺泡纹状管基底膜阳性表达高于大尾羊，eNOS在藏绵羊与大尾羊浆液性腺泡、纹状管内均呈阳性表达，三者在黏液性腺泡均无表达；统计学分析表明，藏绵羊颌下腺EGF与eNOS表达极显著低于大尾羊（P<0.01），EGFR则显著高于大尾羊（P<0.05）。高原牧区藏绵羊颌下腺以浆液性腺泡为主，EGF主要在纹状管分布，EGF与EGFR结合部位主要在纹状管基底膜处，在浆液性腺泡细胞增殖及分泌等方面发挥作用。兰州大尾羊颌下腺内eNOS表达较高且在浆液细胞及纹状管上皮细胞呈强阳性表达可能与高寒低氧环境中应激反应有关。

Abstract: In order to analyze the physiological function of submandibular gland of ruminant and provide information about histomorphology, we compared the histologic structure and the characteristic expression of EGF, EGFR and eNOS between Tibetan sheep and Big-tail sheep living in Tibet Plateau pastoral areas in this study. The tissue structure of submandibular gland and the localization and distribution of EGF, EGFR and eNOS in Tibetan sheep and Big-tail sheep were observed by special staining method, immunohistochemical SP method and immunofluorescence method. Results were as follows:The proportion of serous acinus in Tibetan sheep submandibular gland was larger than that in Big-tail sheep. The AB, PAS and AB-PAS staining were mainly positive in mucinous acinus and mixed gonadal vesicle but not in serous acinus. The expression of EGF in striated tube, intercalated duct epithelial cells and serous acinus of Tibetan sheep were significantly lower than that of Big-tail sheep, and the positive expression of EGFR in submandibular gland of Tibetan sheep was higher than that of Big-tail sheep. the eNOS was positive in serous acinus and striated duct in both of the two kinds of sheep. However, EGF, EGFR and eNOS were expressed negative in mucinous acinus. Statistical analysis showed that the expression levels of EGF and eNOS in Tibetan sheep were significantly lower than that in Big-tail sheep (P<0.01), while the EGFR in Tibetan sheep was significantly greater than that in Big-tail sheep in serous acinar striated tube basement membrane (P<0.05). Taken together, we suggest that the submandibular gland in Tibetan sheep mainly contain serous acinus and strongly positive expression of EGF in submandibular gland of both two kinds of sheep in striated duct basilar membrane, indicating that EGF is mainly secreted in striated duct and the binding sites of EGF and EGFR are mainly in striated duct basilar membrane. It plays an important role in proliferation and secretion of serous acinar cells. Furthermore, the eNOS showing strongly positive expression in both serous cells and striatum epithelial cells may be related to stress reaction under cold and low oxygen environment.

中图分类号:

S852.162

杨婕, 袁莉刚, 宋笑, 王乾梅. 高原牧区藏绵羊与兰州大尾羊颌下腺组织学特征比较[J]. 畜牧兽医学报, 2019, 50(10): 2119-2128.

YANG Jie, YUAN Ligang, SONG Xiao, WANG Qianmei. Comparison of Histologic Characteristics in Submandibular Gland between Tibetan Sheep and Big-tail Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(10): 2119-2128.

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